Detection of corynespora cassiicola in hevea rubber tree. On cotton, it begins a small brickred spot which then leads to the formation of large 14 to 1in. Diversity of corynespora cassiicola isolates and changes. Two pathogens were isolated from tomatoes with severe chlorosis and necrosis in northwestern argentina. Corynespora cassiicola was pathogenic on the roots and hypocotyls of soybean seedlings grown in infested soil at ottawa. Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in malaysia hsueh, 2011. Few studies referred to the formation of chlamydospores by c. General information about corynespora cassiicola coryca. Extensive superficial necrotic lesions developed on seedlings from inoculated seeds planted in the field in midmay, but few symptoms appeared on seedlings planted in late may and in june. Abstract the fungus corynespora cassiicola, causal agent of target spot in soybeans, can. The fungus causing target spot disease, corynespora cassiicola berk.
Structural analysis of cassiicolin, a hostselective. Development of a lamp method for detecting the n75s mutant. However, the underlying factors that contribute to the. This pathogen is common on a wide range of host plants 1,2,6,7,9,and is considered a predominantly tropical pathogen despite its common occurrence as a leaf, stem, and. Corynespora leaf fall, caused by corynespora cassiicola, is one of the most important diseases in rubber hevea brasiliensis plantations. Soybean target leaf spot, caused by the fungus corynespora cassiicola, is controlled especially by leaf application of fungicides. Corynespora cassiicola and stemphylium lycopersici.
Only a single, historical clinical case of human infection caused by c. We report a case of subcutaneous phaeohyphomycosis caused by a plant pathogen in a patient with acute heart failure. In the rubber tree, this fungus causes the corynespora leaf fall clf disease, which increasingly affects natural rubber production in asia and africa. In other words, the leaf wetness is likely a major environmental factor driving the disease for this fungal pathogen.
Wei from infected rubber leaves in sungai buloh two isolates and serdang two isolates were cultured on potato dextrose agar pda. A boll rot of cotton caused by corynespora cassiicola in. Cookies are small text files that contain a string of characters and uniquely identifies a browser. A study was conducted to analyse the diversity among c.
The fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species. Corynespora cassiicola is a common pathogen of grasses and other herbaceous plants in the tropics 3,4. Frontiers compounds released by the biocontrol yeast. Genomewide analysis of corynespora cassiicola leaf fall. The humidity may be used as a surrogate for leaf wetness, as it is an essential component of an environment and has. Pdf symptomatology, hosts, and pathogenicity of corynespora. Wei type species of the genus 100 described species found on diverse substrates. Pdf pathogenicity of corynespora cassiicola on different hosts in. Crops that suffer the greatest yield loss due to the fungus include cowpea, cucumber, papaya, pepper, rubber, soybean and tomato. Pathogenicity of corynespora cassiicola on different hosts in amazonas state. Conner, alabama cooperative extension system, auburn university, al 36849. Most browsers are initially set up to accept cookies, since this is required by most website owners in order to access their sites. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer.
It is not yet known whether the same strain of this pathogen attacks both the crops. Cultural and reproductive variability of corynespora. One of the major fungal plant diseases in the tropical and subtropical regions of the world is corynespora cassiicola. It attacks several plant species including soybean. The isolate, which on cotton is pathogenic only to bolls, is considered to be different from a strain ofc. A new foliar disease, corynespora leaf spot, has been. Pdf sensitivity of corynespora cassiicola from soybean. It is widely used as a medicine against liver and digestion ailments in brazil. Development of a lamp method for detecting the n75s mutant in sdhiresistant corynespora cassiicola. Morphological and inter simple sequence repeat issr. Phaeohyphomycosis caused by a plant pathogen, corynespora. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole article pdf available in tropical plant pathology 385.
Corynespora cassiicola requires high humidity for infection and is additionally favored in locations with substantial periods of high moisture 1644 hours. Corynespora leaf spot or target spot is caused the fungus corynespora cassiicola, which causes leaf spots on cucumber, sweet potato, soybean, tomato and cotton. Genetic variation of 42 isolates of corynespora cassiicola, a destructive fungal pathogen of many economically important crop plants including rubber, was. First report of corynespora cassiicolaincited target spot on cotton in alabama.
Corynespora cassiicola infection is common in plants, but the human corynespora cassiicola infection in our report is rare according to the literature. Author links open overlay panel jiamei zhu a b 1 lingyan zhang a b 1 hong li a b yangyang gao a b wei mu a b feng. The objective of this study was to test the formation of chlamydospores by. Corynespora cassiicola corynespora cassiicola berk.
Chemical control of the soybean target spot corynespora. Saprophytism and parasitism habits of plants are common survival strategies for this pathogen. Chase the first report of a corynespora leaf spot of a foliage plant was made in 1973 on zebra plant aphelandra squarrosa 4. The causal organism was isolated in pure culture and identified ascorynespora cassiicola berk. Corynespora cassiicola, stemphylium lycopersici, chlorosis, fungal diseases of plants, necrosis, plant pathogenic fungi, tomatoes, argentina abstract. Corynespora leaf blight of cotton in brazil and its management. The organism was isolated and identified as corynespora cassiicola according to its. The purpose of this research is to find the reliable means to determine the risk posed to agriculture in the pacific basins by the. Wei was greatly enhanced when the cultures were first scraped after 3 days growth and then grown for an additional 3 days in continuous light. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole sheila a.
Corynespora cassiicola is an ascomycetes fungus with a broad host range and diverse life styles. The necrotrophic fungus corynespora cassiicola berk. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. Corynespora cassiicola is the causal organism of the most devastating leaf disease of rubber in sri lanka. Synthesis and characterization of 11chlorocyclopropyl. Hofs inhibit corynespora cassiicola growth in vitro and protect glycine max plants against this pathogen. Download pdf abstractexcerpt full text pdf contributor notes. Diagnostic features of corynespora cassiicola and its. The isolation of um 591 from a patients contact lens indicates the pathogenic potential of this dematiaceous fungus in human. A total of 618 isolates of corynespora leaf spot fungus corynespora cassiicola collected from 24 commercial cucumber greenhouses in 12 cities in ibaraki prefecture, japan, were tested for their sensitivity to boscalid.
Corynespora cassiicola causing leaf spots on coleus. Distribution and molecular characterization of corynespora. Conventional pcr for detection of corynespora cassiicola. In this study, iprodione ec 50 values ranged from 0. Sensitivity loss by corynespora cassiicola, isolated from.
Cca corynespora blight and target spot corynespora cassiicola gc powdery mildew golovinomyces cichoracearum ccu scab and gummosis cladosporium cucumerinum kgmmv kyuri green mottle mosaic virus cgmmv cucumber green mottle mosaic virus pcu downy mildew pseudoperonospora cubensis cmv cucumber mosaic virus prsv papaya ringspot. Transformation of corynespora cassiicola by agrobacterium tumefaciens. They are sent to a computer by website operators or third parties. Sobers the first report of corynespora cassiicola berk. Their structures including absolute configurations were determined by. Transformation of corynespora cassiicola by agrobacterium. Corynespora cassiicola on chilli in west bengal, india. In recent years, corynespora leaf blight is on the increase in cotton and has become an important disease in the state of mato grosso, brazil. To the best of our knowledge, this study represents the first report of the sensitivity of previously unexposed greenhouse collected isolates of c. Two new naphthalenones, corynenones a and b 1 and 2, and one new depsidone, corynesidone e 3, together with one known depsidone, corynesidone a 4 and two known diphenyl ethers, corynethers a 5 and b 6, were isolated from the spongederived fungus corynespora cassiicola xs20090i7. It is a member of the mitosporic ascomycota, a heterogeneous group of ascomycotic fungi whose common characteristic is the absence of a sexual state. Diagnostic features of corynespora cassiicola and its associated diseases identifying c. Naphthalenones and depsidones from a spongederived.
Morphological features and inter simple sequence repeat issr polymorphism were employed to analyse 21 corynespora cassiicola isolates obtained from a number of hevea clones grown in rubber plantations in malaysia. Corynespora cassiicola, which causes corynespora leaf fall disease, is one of the most destructive and economically important fungal pathogens of hevea rubber trees in asian and african countries. It has been reported in more than 70 host plants including economically important crops such as rubber tree, tomato, cucumber, cotton, soybean, tobacco, cocoa, cowpea,1. On the inoculated stem there are sunken, pale greyish, elliptical lesions, measuring 1. Zhang, department of entomology and plant pathology, auburn university, al 36849. Sensitivity of corynespora cassiicola from soybean to. Differentiation of corynespora cassiicola and cercospora. First report of corynespora cassiicolaincited target spot. A simple and efficient method to prepare 11chlorocyclopropyl21,2,4triazol1ylethan1one via nucleophilic substitution of 2chloro11chlorocyclopropylethanone and 1,2,4triazole is described. An indirect phenotyping method was developed in order to estimate the susceptibility of rubber tree clonal varieties to corynespora leaf fall clf disease caused by the ascomycete corynespora cassiicola. Characterization of the species corynespora cassiicola and. The humidity may be used as a surrogate for leaf wetness, as it is an essential component of an environment and.
Here we confirm the occurrence of this species as an agent of subcutaneous phaeohyphomycosis. The title compound is the key intermediate required for the synthesis of prothioconazole, a promising agricultural fungicide. Genomic insight into pathogenicity of dematiaceous fungus. Cotton, cowpea, cucumber, eggplant, sesame, soybean, tobacco. All four isolates exhibited variability in colony colour and growth rates on artificial media pda, psa, rlea and wa. Corynespora cassiicola is a species of fungus well known as a plant pathogen. Species in the genus induce corynespora leaf spot in bleeding hearts clerodendrum thomsoniae. Frontiers genomewide analysis of corynespora cassiicola. Wei is a cosmopolitan plant pathogen, especially abundant in the tropics. Inter simple sequence repeat issr and rdnaits sequence markers along.
A new boll rot disease of cotton gossypium hirsutum l. Keywords corynespora, leaf spot, chilli capsicum annuum l. He found that the fungus was pathogenic to leaves of hydrangea, hydrangea macrophylla ser. Since that time, corynespora cassiicola has been identified as the cause of leaf spots on weeping fig ficus benjamina 3. Department of plant pathology, bidhan chandra krishi viswavidyalaya. Symptomatology, hosts, and pathogenicity of corynespora cassiicola sintomatologia, hospedeiros e patogenicidade. University of florida, ifas central florida research and education center apopka cfrecapopka research report, rh9312. Corynespora cassiicola causing leaf spots on coleus barbatus corynespora cassiicola causing leaf spots on coleus barbatus fernandes, r. This fungus is present in some hevea rubber plantations and nurseries in hainan and yunnan provinces of south china. The objectives of the present investigation were to verify the genotypic and phenotypic variation between the.
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